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Small Molecule Microarrays: Methods and Protocols: Methods in Molecular Biology, cartea 669

Editat de Mahesh Uttamchandani, Shao Q. Yao
en Limba Engleză Hardback – 21 sep 2010
Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls.Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.
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Specificații

ISBN-13: 9781607618447
ISBN-10: 1607618443
Pagini: 215
Ilustrații: XI, 228 p.
Dimensiuni: 178 x 254 x 15 mm
Greutate: 0.71 kg
Ediția:2010
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Descriere

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls.Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

Cuprins

1. The Expanding World of Small Molecule MicroarraysMahesh Uttamchandani and Shao Q. Yao2. Construction of Photo-Cross-Linked Microarrays of Small MoleculesNaoki Kanoh3. Small Molecule Microarray: Functional-Group Specific Immobilization of Small MoleculesHyang Yeon Lee and Seung Bum Park4. Detecting Protein-Small Molecule Interactions Using Fluorous Small-Molecule MicroarraysArturo J. Vegas and Angela N. Koehler5. A Method for Small Molecule Microarray (SMM)-Based Screening for the Rapid Discovery of Affinity-Based Probes (AfBPs)Haibin Shi, Mahesh Uttamchandani, and Shao Q. Yao6. Nanodroplet Chemical Microarrays and Label-Free AssaysDhaval Gosalia and Scott L. Diamond7. Nanodroplet Microarrays for High-Throughput Enzyme ScreeningKang L.D. Aw, Shao Q. Yao, and Mahesh Uttamchandani8. The Application of the Chemical Array for Biological StudyIsao Miyazaki, Siro Simizu, and Hiroyuki Osada9. Peptide Arrays with a ChipAlexander Nesterov, Edgar Dörsam, Yun-Chien Cheng, Christopher Schirwitz, Frieder Märkle, Felix Löffler, Kai König, Volker Stadler, Ralf Bischoff, and Frank Breitling10. In situ Chemical Modification of Peptide Microarrays: Characterization by Desorption/Ionization on Silicon NanowiresGaëlle Piret, Hervé Drobecq, Rabah Boukherroub, and Oleg Melnyk11. In situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated AntigensRémi Desmet, Eric Diesis, Hervé Drobecq, Carine Rouanet, Karim Chemlal, Anne-Sophie Debrie, Jean-Michel Hougardy, Françoise Mascart, Camille Locht, and Oleg Melnyk12. Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody DetectionMarina Cretich, Francesco Damin, Renato Longhi, Cecilia Gotti, Clelia Galati, Lucio Renna, and Marcella Chiari13. Antibody Signatures Defined by High-Content Peptide Microarray AnalysisAntonia Masch, Johannes Zerweck, Ulf Reimer, Holger Wenschuh, and Mike Schutkowski14. HighDensity Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell LysatesAlexandra Thiele, Matthias Weiwad, Johannes Zerweck, Gunter Fischer, and Mike Schutkowski15. A Peptide Microarray for Detecting Protein Kinase Activity in Cell LysatesXiaoming Han and Yoshiki Katayama16. Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their ApplicationsSungjin Park, Myung-Ryul Lee, and Injae Shin17. A Microarray-Based Method to Perform Nucleic Acid SelectionsOlga Aminova and Matthew D. Disney

Textul de pe ultima copertă

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls.Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

Caracteristici

Covers chemical libraries, peptide libraries, and carbohydrates with contributions from experts in the microarray field -Provides essential methods and skills learned through hands-on experience and feedback at the bench -Includes detailed notes highlighting tips on troubleshooting and avoiding known experimental pitfalls
Includes supplementary material: sn.pub/extras