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Radiation Cytogenetics: Methods and Protocols: Methods in Molecular Biology, cartea 1984

Editat de Takamitsu A. Kato, Paul F. Wilson
en Limba Engleză Hardback – 4 iul 2019
This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.
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Specificații

ISBN-13: 9781493994304
ISBN-10: 1493994301
Pagini: 144
Ilustrații: X, 138 p. 18 illus., 11 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.52 kg
Ediția:1st ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

1. Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis
            Takamitsu A. Kato
 
2. Animal Lymphocyte Metaphase Chromosome Preparation
            Kazuki Heishima, Kendon Kuo, Masashi Kimura, and Takashi Mori
 
3. Micronuclei Formation Analysis after Ionizing Radiation
            Cathy Su, Alexis H. Haskins, and Takamitsu A. Kato
 
4. G1 Premature Chromosome Condensation (PCC) Assay
            Ryuichi Okayasu and Cuihua Liu
 
5. G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity
            Jeremy S. Haskins and Takamitsu A. Kato
 
6. G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology
            Eisuke Gotoh
 
7. Sister Chromatid Exchange as a Genotoxic Stress Marker
            Shigeaki Sunada, Jeremy S. Haskins, and Takamitsu A. Kato
 
8. DNA Damage Focus Formation Assay
            Yoshihiro Fujii
 
9. Nuclear Foci Assays in Live Cells
            Eiichiro Mori and Aroumougame Asaithamby
 
10. In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes
            Chisato Omata and Takamitsu A. Kato
 
11. PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations
            Ian M. Cartwright, Jeremy S. Haskins, and Takamitsu A. Kato
 
12. Modified PNA Telomere and Centromere FISH Protocols
            Ian M. Cartwright
 
13. Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements
            Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey, and F. Andrew Ray
 
14. Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH
            Jeremy S. Haskins and Takamitsu A. Kato
 
15. Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting
            Bradford D. Loucas

Textul de pe ultima copertă

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.

Caracteristici

Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts