Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods: Methods in Molecular Biology, cartea 2178
Editat de Nikolaos E. Labrouen Limba Engleză Hardback – noi 2020
This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry.
Toate formatele și edițiile | Preț | Express |
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Paperback (2) | 711.66 lei 39-44 zile | |
Humana Press Inc. – 3 sep 2016 | 711.66 lei 39-44 zile | |
Springer Us – noi 2021 | 829.01 lei 39-44 zile | |
Hardback (2) | 1002.56 lei 39-44 zile | |
Humana Press Inc. – 20 mar 2014 | 1002.56 lei 39-44 zile | |
Springer Us – noi 2020 | 1235.71 lei 39-44 zile |
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Specificații
ISBN-13: 9781071607749
ISBN-10: 107160774X
Ilustrații: XV, 511 p. 127 illus., 78 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 1.18 kg
Ediția:2nd ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
ISBN-10: 107160774X
Ilustrații: XV, 511 p. 127 illus., 78 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 1.18 kg
Ediția:2nd ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
Cuprins
Preface…
Table of Contents…
Contributing Authors…
Part I Screening and Design Purification Strategies
1. Protein Purification Technologies
Nikolaos E. Labrou
2. High Throughput Process Development: I. Process Chromatography
Anurag S. Rathore and R. Bhambure
3. High Throughput Process Development: II. Membrane Chromatography
Anurag S. Rathore and S. Muthukumar
4. Media Selection in Ion Exchange Chromatography in a Single Microplate
Charlotte Cabanne and Xavier Santarelli
5. High Throughput Screening of Dye-Ligands for Chromatography
Sunil Kumar and Narayan S. Punekar
6. Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers
Peter M. Schmidt
Part II Low-Resolution Protein Purification Methods
7. Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts
Oscar Aguilar, Erick Heredia-Olea, Esther Perez-Carrillo, and Marco Rito-Palomares
8. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins
Anurag S. Rathore and R. Bhambure
9. Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments
Ario de Marco
Part III High-Resolution Protein Purification Methods
10. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview
Ana Sofia Pina, Íris L. Batalha, Ana M. G. C. Dias, and Ana Cecília A. Roque
11. Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins
Sharon Williams, Phil Morton, and Dev Baines
12. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery
My Hedhammar, Johan Nilvebrant, and Sophia Hober
13. An Orthogonal Fusion Tag for Efficient Protein Purification
Johan Nilvebrant, Mikael Åstrand, and Sophia Hober
14. Biomimetic Affinity Ligands for Protein Purification
Isabel T. Sousa and M. Ângela Taipa
15. Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification
Evangelia G. Chronopoulou, Georgios Premetis, Christina Varotsou, Nikolaos Georgakis, Elisavet Ioannou and Nikolaos E. Labrou
16. Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening
G. R. Barredo, S. L. Saavedra, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, O. Cascone, and S. A. Camperi
17. ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification
Julia Scheffel, Sara Kanje, and Sophia Hober
18. Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing
E.G. Korzhikova-Vlakha, G.A. Platonovaa, and T.B. Tennikova
19. Sample Displacement Batch Chromatography of Proteins
Laura Heikaus, Siti Hidaya, Manasia Gaikwad, Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter
20. Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3
Salvatore G. De-Simone and D. William Provance, Jr.
21. Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins
Sanhita Maity, Musaab Al-ameer, Ravi Kumar Gundampati, Shilpi Agrawal, and Thallapuranam Krishnaswamy Suresh Kumar
22. Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+
Jessica J. Gomez-Lugo, Bryan D. Santos, David A. Perez-Perez, Jorge M. Montfort-Gardeazabal, Megan M. McEvoy, and Xristo Zarate
23. Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin
Tao Chen, Gaili Guo, Guoqing Tan, Ying Wang, and Yifeng Li
Part IV Assessing Protein Structural Integrity, Purity, and Stabilization
24. Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry
Cosima D. Calvano, Mariachiara Bianco, Ilario Losito, and Tommaso R.I. Cataldi
25. Protein Structure Analysis and Validation with X-Ray Crystallography
Anastassios C. Papageorgiou, Nirmal Poudel, and Jesse Mattsson
26. Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes
Annette C. Moser, Benjamin White, and Frank A. Kovacs
27. Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin
Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda Dráberová
Part V Applications/Case Studies
28. G Protein-Coupled Receptor Expression and Purification
Karolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos
29. (Hyper)thermophilic Enzymes: Production and Purification
Pierpaolo Falcicchio, Mark Levisson, Servé W.M. Kengen, Sotirios Koutsopoulos, and John van der Oost
30. Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays
Anthi Karnaouri, Anastasia Zerva, Paul Christakopoulos, and Evangelos Topakas
Table of Contents…
Contributing Authors…
Part I Screening and Design Purification Strategies
1. Protein Purification Technologies
Nikolaos E. Labrou
2. High Throughput Process Development: I. Process Chromatography
Anurag S. Rathore and R. Bhambure
3. High Throughput Process Development: II. Membrane Chromatography
Anurag S. Rathore and S. Muthukumar
4. Media Selection in Ion Exchange Chromatography in a Single Microplate
Charlotte Cabanne and Xavier Santarelli
5. High Throughput Screening of Dye-Ligands for Chromatography
Sunil Kumar and Narayan S. Punekar
6. Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers
Peter M. Schmidt
Part II Low-Resolution Protein Purification Methods
7. Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts
Oscar Aguilar, Erick Heredia-Olea, Esther Perez-Carrillo, and Marco Rito-Palomares
8. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins
Anurag S. Rathore and R. Bhambure
9. Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments
Ario de Marco
Part III High-Resolution Protein Purification Methods
10. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview
Ana Sofia Pina, Íris L. Batalha, Ana M. G. C. Dias, and Ana Cecília A. Roque
11. Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins
Sharon Williams, Phil Morton, and Dev Baines
12. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery
My Hedhammar, Johan Nilvebrant, and Sophia Hober
13. An Orthogonal Fusion Tag for Efficient Protein Purification
Johan Nilvebrant, Mikael Åstrand, and Sophia Hober
14. Biomimetic Affinity Ligands for Protein Purification
Isabel T. Sousa and M. Ângela Taipa
15. Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification
Evangelia G. Chronopoulou, Georgios Premetis, Christina Varotsou, Nikolaos Georgakis, Elisavet Ioannou and Nikolaos E. Labrou
16. Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening
G. R. Barredo, S. L. Saavedra, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, O. Cascone, and S. A. Camperi
17. ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification
Julia Scheffel, Sara Kanje, and Sophia Hober
18. Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing
E.G. Korzhikova-Vlakha, G.A. Platonovaa, and T.B. Tennikova
19. Sample Displacement Batch Chromatography of Proteins
Laura Heikaus, Siti Hidaya, Manasia Gaikwad, Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter
20. Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3
Salvatore G. De-Simone and D. William Provance, Jr.
21. Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins
Sanhita Maity, Musaab Al-ameer, Ravi Kumar Gundampati, Shilpi Agrawal, and Thallapuranam Krishnaswamy Suresh Kumar
22. Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+
Jessica J. Gomez-Lugo, Bryan D. Santos, David A. Perez-Perez, Jorge M. Montfort-Gardeazabal, Megan M. McEvoy, and Xristo Zarate
23. Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin
Tao Chen, Gaili Guo, Guoqing Tan, Ying Wang, and Yifeng Li
Part IV Assessing Protein Structural Integrity, Purity, and Stabilization
24. Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry
Cosima D. Calvano, Mariachiara Bianco, Ilario Losito, and Tommaso R.I. Cataldi
25. Protein Structure Analysis and Validation with X-Ray Crystallography
Anastassios C. Papageorgiou, Nirmal Poudel, and Jesse Mattsson
26. Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes
Annette C. Moser, Benjamin White, and Frank A. Kovacs
27. Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin
Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda Dráberová
Part V Applications/Case Studies
28. G Protein-Coupled Receptor Expression and Purification
Karolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos
29. (Hyper)thermophilic Enzymes: Production and Purification
Pierpaolo Falcicchio, Mark Levisson, Servé W.M. Kengen, Sotirios Koutsopoulos, and John van der Oost
30. Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays
Anthi Karnaouri, Anastasia Zerva, Paul Christakopoulos, and Evangelos Topakas
Textul de pe ultima copertă
This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry.
Caracteristici
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts