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Plant Metabolism: Methods and Protocols: Methods in Molecular Biology, cartea 1083

Editat de Ganesh Sriram
en Limba Engleză Paperback – 17 sep 2016
In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.
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Specificații

ISBN-13: 9781493958818
ISBN-10: 149395881X
Pagini: 318
Ilustrații: X, 318 p. 79 illus., 43 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.57 kg
Ediția:Softcover reprint of the original 1st ed. 2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Part I: Metabolites and Metabolic Fluxes
1. MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism
Catherine Deborde and Daniel Jacob
 
2. Targeted Deuteration of Polyphenolics for their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived Extracts
Mikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman
 
3. Relative Quantitation in Single Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry
Bindesh Shrestha and Akos Vertes
 
4. Quantification of Plant Volatiles
Anthony V. Qualley and Natalia Dudareva
 
5. Quantitative Imaging Approaches for Small Molecule Measurements using FRET Sensors in Plants
Sakiko Okumoto
 
6. Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance
Quyen X. Truong, Jong Moon Yoon, and  Jacqueline V. Shanks
 
7. Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry
Jamey D. Young, Douglas K. Allen, and John A. Morgan
 8. Mathematical Modeling of Isotope Labeling Experiments for Metabolic Flux Analysis Shilpa Nargund and Ganesh Sriram
 
9. Optimal Design of Isotope Labeling Experiments
Hong Yang, Dominic E Mandy and Igor GL Libourel
 
Part II: Metabolic Pathways and Metabolic Systems Biology
10. Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities
Kate Dreher
 
11. Elucidation of Metabolic Pathways from Enzyme Classification Data
Andrew G. McDonald and Keith F. Tipton
 
12. Deducing Intracellular Distributions of Metabolic Pathways from Genomic Data
Ansgar Gruber and Peter G. Kroth
 13. Genome-scale Models of Plant Metabolism Margaret Simons, Ashish Misra, and Ganesh Sriram
 
14. Elementary Flux Modes, Flux Balance Analysis and their Application to Plant Metabolism Katrin Lotz, Anja Hartmann, Eva Grafahrend-Belau, Falk Schreiber, and Björn H. Junker
 
15. Systems Approaches to Unraveling Plant Metabolism: Identifying Biosynthetic Genes of Secondary Metabolic Pathways
Martin J. Spiering, Bhavneet Kaur, James F. Parsons, and Edward Eisenstein
 
16. Applications of Kinetic Modeling to
Plant Metabolism
Johann M. Rohwer
 
17. Kinetic Modeling of Plant Metabolism and its Predictive Power – Peppermint Essential Oil Biosynthesis as an Example
Bernd Markus Lange and Rigoberto Rios-Estepa
 

Textul de pe ultima copertă

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

Caracteristici

Details disciplines ranging from plant biology to metabolic engineering and biotechnology
Provides step-by-step details essential for reproducible results
Contains valuable notes and implementation advice from the experts