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Non-Viral Gene Delivery Vectors: Methods and Protocols: Methods in Molecular Biology, cartea 1445

Editat de Gabriele Candiani
en Limba Engleză Hardback – 20 iul 2016
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery—also termed reversetransfection—and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.
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Specificații

ISBN-13: 9781493937165
ISBN-10: 1493937162
Pagini: 170
Ilustrații: XIV, 290 p. 66 illus., 36 illus. in color.
Dimensiuni: 178 x 254 x 19 mm
Greutate: 0.75 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

 

Part I: Conventional Bolus Gene Delivery Vectors
1. Physical Chemical and BiomolecularMethods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitrofor the Gene Therapy Applications

 

 

Santosh K. Misra and Santanu Bhattacharya

2. Cationic Lipid-Based Nucleic Acid Vectors
Emile Jubeli, William P. D. Goldring, and Michael D.Pungente

3. Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPELiposomes and Gemini Micelles
Sara Falsini and Sandra Ristori

4. Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA
AnaL. Barrán-Berdón, Emilio Aicart,  andElena Junquera

5. Elaboration and Physicochemical Characterization of Niosome-BasedNioplexes for Gene Delivery Purposes
EdilbertoOjeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, JonZarate, and Jose L. Pedraz

6. Quantitative Intracellular Localization of Cationic Lipid-NucleicAcid Nanoparticles with Fluorescence Microscopy
Ramsey N.Majzoub, Kai K. Ewert, and Cyrus R. Safinya

7. TargetedDelivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells
Mario Ariatti

8. Lipoplexes Strengthened by AnionicPolymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipidsand Anionic Polymers
Danielle Arruda, Anne Schlegel, Pascal Bigey, and VirginieEscriou

9. Polymer Based Gene Silencing: In Vitro Delivery of SiRNA
MargaridaI. Simão Carlos, Andreas Schätzlein, and Ijeoma Uchegbu

10. PolyallylamineDerivatives: Novel Non-Toxic Transfection Agents
MagdalenaWytrwal and Chantal Pichon

11. BiodegradableThree-Layered Micelles and Injectable Hydrogels
Daniel G.Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Tomoko Fujiwara, andOlivia M. Merkel

12. Cationic Lipid-CoatedPolyplexes (“Lipopolyplexes”) for DNA and Small RNA Delivery
AlexanderEwe and Achim Aigner

13. Preparation of Targeted AnionicLipid-Coated Polyplexes for MicroRNA Delivery
XiaomengHuang, Mengzi Zhang, Xinmei Wang, L. James Lee, and Robert J. Lee

Part II: Stimuli-Responsive Bolus Gene Delivery Vectors

14. Characterizationand Investigation of Redox-Sensitive Liposomes for Gene Delivery
Daniele Pezzoli, ElenaTallarita, Elena Rosini, and Gabriele Candiani

15. From ArtificialAmino Acids to Sequence-Defined Targeted Oligoaminoamides
StephanMorys, Ernst Wagner, and Ulrich Lächelt

16. Gene DeliveryMethod Using Photo-Responsive Poly(β-Amino Ester) as Vectors
NanZheng, Yang Liu, and Jianjun Cheng

17. Thermo-ResponsivePolyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for SystemicGene Therapy
Junjie Li, Zengshi Zha, andZhishen Ge

Part III: Substrate-Mediated Gene Delivery

18. Application of Polyethylenimine-Grafted Silicon NanowireArrays for Gene Transfection
Hongwei Wang, Jingjing Pan, Hong Chen, and Lin Yuan
 


Textul de pe ultima copertă

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery—also termed reversetransfection—and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.


Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Descriere

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery—also termed reversetransfection—and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.