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Neurotrophic Factors: Methods and Protocols: Methods in Molecular Biology, cartea 846

Editat de Stephen D. Skaper
en Limba Engleză Hardback – 25 feb 2012
Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.
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Specificații

ISBN-13: 9781617795350
ISBN-10: 1617795356
Pagini: 400
Ilustrații: XIV, 421 p.
Dimensiuni: 178 x 254 x 30 mm
Greutate: 0.95 kg
Ediția:2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

1. The Neurotrophin Family of Neurotrophic Factors – An Overview
            Stephen D. Skaper
 
2. Neuronal Growth-Promoting and Inhibiting Cues in Neuroprotection and Neurodegeneration
            Stephen D. Skaper
 
3. Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents
            Laura Facci and Stephen D. Skaper
 
4. Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum
            Morena Zusso and Patrizia Debetto
 
5. Culture of Rodent Cortical and Hippocampal Neurons
            Laura Facci and Stephen D. Skaper
 
6. Amyloid β-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons
            Laura Facci and Stephen D. Skaper
 
7. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord
            Stephen D. Skaper, Carla Argentini, and Massimo Barbierato
 
8. Central Nervous System Neuron-Glia Co-Culture Models
            Stephen D. Skaper and Laura Facci
 
9. Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons
            Stephen D. Skaper, Giulia Mercanti, and Laura Facci
 
10. Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions
            Jose V. Montoya-Gacharna, Jhon Jairo Sutachan, Wai Si Chan, Alexandra Sideris, Thomas J.J. Blanck, and Esperanza Recio-Pinto
 
11. Rodent Retinal Ganglion Cell Cultures
            Stephen D. Skaper
 
12. Culture of Purified Glial Cell Populations from Optic Nerve
            Stephen D. Skaper
 
13. Isolation and Culture of Rat Cone Photoreceptor Cells
            Stephen D. Skaper
 
14. Rat Retina Pigmented Epithelial Cells
            Stephen D. Skaper
 
15. Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms that Underlie Axon Growth, Guidance and Regeneration
            Andrew J. Murray, Andrew G. Peace, Steven J. Tucker  and Derryck A. Shewan
 
16. Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats
            Davina E. Owen and Julie Egerton
 
17. Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man
            Kirsten Haastert-Talini
 
18. Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson’s Disease
            Cristina Malagelada Grau and Lloyd A. Greene
 
19. Compartmented Chambers for Studying Neurotrophic Factor Action
            Stephen D. Skaper
 
20. Preparation and Culture of Adrenal Chromaffin Cells
            Natalia Domínguez, Miriam Rodríguez, J. David Machado, and Ricardo Borges
 
21. Indirect Immunofluorescence Staining of Cultured Neural Cells
            Massimo Barbierato, Carla Argentini, and Stephen D. Skaper
 
22. Neurite Outgrowth Assessment Using High Content Analysis Methodology
            Nicholas M. Radio
 
23. Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth
            William Lee and Vladimir Parpura
 
24. High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures
            Frederik Sündermann, Nataliya Golovyashkina, Roland Brandt, Lidia Bakota, and Christian Tackenberg
 
25. Imaging Amyloid Precursor Protein In Vivo – An Axonal Transport Assay
            Tomás L. Falzone and Gorazd B. Stokin
 
26. The Use of Specific AAV Serotypes to Stably Transduce  Primary CNS Neuron Cultures
            Saafan Z. Malik, Margie Maronski, Marc A. Dichter and Deborah J. Watson
 
27. Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides
            Secil Caban, Yilmaz Capan, Patrick Couvreur, and Turgay Dalkara
 
28.  [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells
            Stephen D. Skaper and Laura Facci
 
29. Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents
            Elisabetta Gerace, Elisa Landucci, Tania Scartabelli, Flavio Moroni, and Domenico E. Pellegrini-Giampietro
 
30. A 6-Hydroxydopamine In Vivo Model of Parkinson’s Disease
            Giulia Mercanti, Gianfranco Bazzu, and Pietro Giusti
 
31. Brain Microdialysis in Freely Moving Animals
            Gianfranco Bazzu, Alice Biosa, Donatella Farina, Ylenia Spissu, Giammario Calia, Sonia Dedola, Gaia Rocchitta, Rossana Migheli, Pier Andrea Serra, and Maria Speranza Desole
 
32. Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion
            Andre Schwambach Vieira, Alexandre Cesar Santos de Rezende, and Fabio Rogerio
 
33. Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection
            Hari Shanker Sharma and Aruna Sharma

Recenzii

From the reviews:
“It provides a foundation for neurobiological research into the effects of neurotrophic factors and other chemicals on the nervous system. … The audience is broad, including practicing and in training neuroscientists, neurobiologists, and basic neuroscience researchers in human and veterinary medicine, as well as academic neurologists, neurosurgeons, and pharmacists. The methods can be incorporated into basic research laboratories and into medical, biology, and veterinary classes.” (Ramsis Farid Ghaly, Doody’s Book Reviews, July, 2012)

Textul de pe ultima copertă

Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.

Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts