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Membrane Biogenesis: Methods and Protocols: Methods in Molecular Biology, cartea 1033

Editat de Doron Rapaport, Johannes M. Herrmann
en Limba Engleză Paperback – 23 aug 2016
Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
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Specificații

ISBN-13: 9781493963041
ISBN-10: 149396304X
Pagini: 418
Ilustrații: XIV, 404 p. 80 illus., 48 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 7.84 kg
Ediția:Softcover reprint of the original 1st ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Part I: Investigation of Membrane Lipids
 
1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
            Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger
 
2. Thin-Layer Chromatography of Phospholipids
            Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg
 
3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
            Martina Gsell and Günther Daum
 
Part II: Analysis of Structure and Topology of Membrane Proteins
 
4. Using 2D Crystals to Analyze the Structure of Membrane Proteins
            Ian Collinson, Janet Vonck, and Dilem Hizlan
 
5. Crystallization of Membrane Proteins
            Florian G. Müller and C. Roy D. Lancaster
 
6. Molecular Dynamics Simulations of Membrane Proteins
            Kristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann
 
7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins
            Christine T. Schwall and Nathan N. Alder
 
8. Topology Determination of Untagged Membrane Proteins
            Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner
 
9. selfassembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
            Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. Sommer
 
Part III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes
 
10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane
            Milit Marom and Abdussalam Azem
 
11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance
            Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen
 
12. Peptide Interaction with and Insertion into Membranes
            Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai
 
13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
            Joseph D. Unsay and Ana J. García-Sáez
 
14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast
            Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo
 
15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins
            Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel
 
16. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry
            Carolyn Vargas, Johannes Klingler, and Sandro Keller
 
Part IV: Membrane Biogenesis and Dynamics
 
17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria
            Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann
 
18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells
            Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann
 
19. An Assay to Monitor the Membrane Integration of Single-Span Proteins
            Katrin Krumpe and Doron Rapaport
 
20. Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
            Daniel Weckbecker and Johannes M. Herrmann
 
Part V: Functional Analysis of Membrane Proteins
 
21. Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
            Martin van der Laan, Ralf M. Zerbes, and Chris van der Does
 
22. Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes
            Philipp Bartsch, Anke Harsman, and Richard Wagner
 
23. Quantification of Protein Complexes by Blue Native Electrophoresis
            Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, and Ilka Wittig
 
24. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
            Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew, and Jan-Willem de Gier

Textul de pe ultima copertă

Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.

Caracteristici

Features numerous techniques to study membrane proteins and membrane lipids as well as their interactions
Includes detailed, lab-ready protocols and methodologies
Contains expert tips and key implementation advice to ensure successful results