JAK-STAT Signalling: Methods and Protocols: Methods in Molecular Biology, cartea 967
Editat de Sandra E. Nicholson, Nicos A. Nicolaen Limba Engleză Hardback – 8 ian 2013
Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
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Humana Press Inc. – 8 ian 2013 | 538.74 lei 39-44 zile |
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Specificații
ISBN-13: 9781627032414
ISBN-10: 162703241X
Pagini: 332
Ilustrații: XI, 320 p.
Dimensiuni: 178 x 254 x 23 mm
Greutate: 0.75 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 162703241X
Pagini: 332
Ilustrații: XI, 320 p.
Dimensiuni: 178 x 254 x 23 mm
Greutate: 0.75 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Public țintă
Professional/practitionerCuprins
Part I. JAK Protein Tyrosine Kinases
1. Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
Jeremy A. Ross, Rodriguez Georgialina, and Robert A. Kirken
2. Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins
Claude Haan and Serge Haan
3. In Vitro JAK Kinase Activity and Inhibition Assays
Jeffrey J. Babon and James M. Murphy
4. Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance
Jeffrey J. Babon
5. Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach
Anna Dittrich, Elmar Siewert, and Fred Schaper
6. Designing RNAi Screens to Identify JAK/STAT Pathway Components
Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler
7. The Use of JAK-Specific Inhibitors as Chemical Biology Tools
Christopher J. Burns, David Segal, and Andrew F. Wilks
8. Methods for Detecting Mutations in the Human JAK2 Gene
Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green
Part II. Signal Transducers and Activators of Transcription (STAT)s
9. Detection of Activated STAT Proteins
Jean-Patrick Parisien and Curt M. Horvath
10. Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents
Serge Jaan and Claude Haan
11. Flow Cytometric Analysis of STAT Phosphorylation
Jane Murphy and Gabrielle L. Goldberg
12. Acetylation of Endogenous STAT Proteins
Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer
13. Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
Reza Krotooshi and Trevor Owens
14. Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
Velasco Cimica and Nancy C. Reich
15. Characterization of STAT Self-Association by Analytical Ultracentrifugation
Nikola Wenta and Uwe Vinkemeier
16. Constitutively Active STAT5 Constructs
Lynn M. Heltemes-Harris and Michael A. Farrar
Part III. Suppressor of Cytokine Signalling (SOCS) Proteins
17. Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
Tatiana B. Kolesnik and Sandra E. Nicholson
18. Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
Jian-Guo Zhang and Sandra E. Nicholson
19. In vitro Ubiquitination of Cytokine Signaling Components
Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw
Part IV. Structural Analysis of the JAK/STAT Pathway
20. Production and Crystallization of Recombinant JAK Proteins
Isabelle S. Lucet and Rebecca Bamert
21. Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins
Florence Baudin and Christoph W. Müller
1. Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
Jeremy A. Ross, Rodriguez Georgialina, and Robert A. Kirken
2. Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins
Claude Haan and Serge Haan
3. In Vitro JAK Kinase Activity and Inhibition Assays
Jeffrey J. Babon and James M. Murphy
4. Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance
Jeffrey J. Babon
5. Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach
Anna Dittrich, Elmar Siewert, and Fred Schaper
6. Designing RNAi Screens to Identify JAK/STAT Pathway Components
Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler
7. The Use of JAK-Specific Inhibitors as Chemical Biology Tools
Christopher J. Burns, David Segal, and Andrew F. Wilks
8. Methods for Detecting Mutations in the Human JAK2 Gene
Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green
Part II. Signal Transducers and Activators of Transcription (STAT)s
9. Detection of Activated STAT Proteins
Jean-Patrick Parisien and Curt M. Horvath
10. Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents
Serge Jaan and Claude Haan
11. Flow Cytometric Analysis of STAT Phosphorylation
Jane Murphy and Gabrielle L. Goldberg
12. Acetylation of Endogenous STAT Proteins
Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer
13. Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
Reza Krotooshi and Trevor Owens
14. Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
Velasco Cimica and Nancy C. Reich
15. Characterization of STAT Self-Association by Analytical Ultracentrifugation
Nikola Wenta and Uwe Vinkemeier
16. Constitutively Active STAT5 Constructs
Lynn M. Heltemes-Harris and Michael A. Farrar
Part III. Suppressor of Cytokine Signalling (SOCS) Proteins
17. Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
Tatiana B. Kolesnik and Sandra E. Nicholson
18. Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
Jian-Guo Zhang and Sandra E. Nicholson
19. In vitro Ubiquitination of Cytokine Signaling Components
Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw
Part IV. Structural Analysis of the JAK/STAT Pathway
20. Production and Crystallization of Recombinant JAK Proteins
Isabelle S. Lucet and Rebecca Bamert
21. Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins
Florence Baudin and Christoph W. Müller
Textul de pe ultima copertă
The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
Caracteristici
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras