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JAK-STAT Signalling: Methods and Protocols: Methods in Molecular Biology, cartea 967

Editat de Sandra E. Nicholson, Nicos A. Nicola
en Limba Engleză Hardback – 8 ian 2013
The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
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Specificații

ISBN-13: 9781627032414
ISBN-10: 162703241X
Pagini: 332
Ilustrații: XI, 320 p.
Dimensiuni: 178 x 254 x 23 mm
Greutate: 0.75 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Part I. JAK Protein Tyrosine Kinases
 
1. Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
                Jeremy A. Ross, Rodriguez Georgialina, and Robert A. Kirken
 
2. Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins
                Claude Haan and Serge Haan
 
3. In Vitro JAK Kinase Activity and Inhibition Assays
                Jeffrey J. Babon and James M. Murphy
 
4. Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance
                Jeffrey J. Babon
 
5. Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach
                Anna Dittrich, Elmar Siewert, and Fred Schaper
 
6. Designing RNAi Screens to Identify JAK/STAT Pathway Components
                Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler
 
7. The Use of JAK-Specific Inhibitors as Chemical Biology Tools
                Christopher J. Burns, David Segal, and Andrew F. Wilks
 
8. Methods for Detecting Mutations in the Human JAK2 Gene
                Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green
 
Part II. Signal Transducers and Activators of Transcription (STAT)s
 
9. Detection of Activated STAT Proteins
                                Jean-Patrick Parisien and Curt M. Horvath
 
10. Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents
                Serge Jaan and Claude Haan
 
11. Flow Cytometric Analysis of STAT Phosphorylation
                Jane Murphy and Gabrielle L. Goldberg
 
12. Acetylation of Endogenous STAT Proteins
                Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer
 
13. Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
                Reza Krotooshi and Trevor Owens
 
14. Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
                Velasco Cimica and Nancy C. Reich
 
15. Characterization of STAT Self-Association by Analytical Ultracentrifugation
                Nikola Wenta and Uwe Vinkemeier
 
16. Constitutively Active STAT5 Constructs
                Lynn M. Heltemes-Harris and Michael A. Farrar
 
Part III. Suppressor of Cytokine Signalling (SOCS) Proteins
 
17. Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
                Tatiana B. Kolesnik and Sandra E. Nicholson
 
18. Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
                Jian-Guo Zhang and Sandra E. Nicholson
 
19. In vitro Ubiquitination of Cytokine Signaling Components
                Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw
 
Part IV. Structural Analysis of the JAK/STAT Pathway
 
20. Production and Crystallization of Recombinant JAK Proteins
                Isabelle S. Lucet and Rebecca Bamert
 
21. Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins
                Florence Baudin and Christoph W. Müller

Textul de pe ultima copertă

The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.

Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras