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Imaging Flow Cytometry: Methods and Protocols: Methods in Molecular Biology, cartea 1389

Editat de Natasha S. Barteneva, Ivan A. Vorobjev
en Limba Engleză Hardback – 23 noi 2015
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
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Specificații

ISBN-13: 9781493933006
ISBN-10: 1493933000
Pagini: 265
Ilustrații: XIII, 295 p. 89 illus., 86 illus. in color.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.76 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

Part I: General
 
1. Quantitative Functional Morphology by Imaging Flow Cytometry
            Ivan A. Vorobjev and Natasha S. Barteneva
 
2. Principles of Amnis Imaging Flow Cytometry
            David A. Basiji
 
3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch
            Andy K.S. Lau, Terence T.W. Wong, Ho Cheung Shum, Kenneth K.Y. Wong, and Kevin K. Tsia
 
4. Applications of Imaging Flow Cytometry for Microalgae
            Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith, Roshan P. Shrestha, Orna Cook, Eva Sanchez, Kalpana Manandhar-Shrestha, and Benjamin Alderete
 
Part II: IFC in Detection of Nuclei and Nucleic Acids
 
5. The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry
            Andrew Filby, William Day, Sukhveer Purewal, and Nuria Martinez-Martin
 
6. Quantitation of Chromosome Damage by Imaging Flow Cytometry
            Lindsay A. Beaton-Green and Ruth C. Wilkins
 
7. Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry
            Orla Maguire, Paul K. Wallace, and Hans Minderman
 
8. Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry
            Elizaveta Fasler-Kan, Yeldar Baiken, Ivan Vorobjev, and Natasha S. Barteneva
 
9. Usin
g Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation             Adam S. Venable, Andrea L. Henning, Eric A. Prado, and Brian K. McFarlin
 
Part III: IFC in Immune Response and Drug Screening
 
10. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy
            Guido H. Wabnitz and Yvonne Samstag
 
11. Studying T Cells N-Glycosylation by Imaging Flow Cytometry
            Ana M. Dias, Catarina R. Almeida, Celso A. Reis, and Salomé S. Pinho
 
12. Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry
            Brian K. McFarlin, Eric A. Prado, Adam S. Venable, Andrea L. Henning, and Randall R. Williams
 
13. Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity
            Andrea L. Henning, Adam S. Venable, Eric A. Prado, and Brian K. McFarlin
 
14. Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry
            Yan Quan Lee, Brian E.Hall, and Kevin S.W. Tan
 
Part IV: IFC in Studies of Cell Death and Apoptosis
 
15. Accurate Assessment of Cell Death by Imaging Flow Cytometry
            Aja M. Rieger and Daniel R. Barreda
 
16. Temporal Heterogeneity
in Apoptosis Determined by Imaging Flow Cytometry             Ivan A. Vorobjev and Natasha S. Barteneva
 
Part V: Imaging Cytometry in Cell Classification and Morphological Analysis
 
17. FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry
            Nicole J. Poulton
 
18. Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry
            Leigh Samsel and J. Philip McCoy, Jr.
 
19. Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes
            Lisa M. Niswander, James Palis, and Kathleen E. McGrath
 
20. Sickle Cell Imaging Flow Cytometry Assay (SIFCA)
            Kleber Y. Fertrin, Leigh Samsel, Eduard J. van Beers, Laurel Mendelsohn, Gregory J. Kato, and J. Philip McCoy

Recenzii

“The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and protocols. The authors are successful in achieving these worthy objectives. Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications. … This book for the first time explores the techniques and protocols involving quantitative imaging flow cytometry in a fascinating manner.” (Omer Iqbal, Doody's Book Reviews, September, 2016)

Textul de pe ultima copertă

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Caracteristici

Includes cutting-edge methods and protocols for imaging flow cytometry
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts