Cantitate/Preț
Produs

High Throughput Screening: Methods and Protocols: Methods in Molecular Biology, cartea 1439

Editat de William P. Janzen
en Limba Engleză Paperback – 31 mai 2018
This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening,  high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 50611 lei  39-44 zile
  Springer – 31 mai 2018 50611 lei  39-44 zile
Hardback (1) 98126 lei  39-44 zile
  Springer – 18 iun 2016 98126 lei  39-44 zile

Din seria Methods in Molecular Biology

Preț: 50611 lei

Preț vechi: 62483 lei
-19%

Puncte Express: 759

Preț estimativ în valută:
9696 10527$ 8315£

Carte tipărită la comandă

Livrare economică 06-11 mai

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493981137
ISBN-10: 1493981137
Ilustrații: XI, 308 p. 101 illus., 71 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.61 kg
Ediția:Softcover reprint of the original 3rd ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

1. Design and Implementation of High Throughput Screening Assays
David J. Powell, Robert P. Hertsberg,and Ricardo Macarrόn

2. Characterization of InhibitorBinding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method
Thomas V. Riera, Tim J. Wigle, andRobert A. Copeland

3. High Throughput Screening UsingMass Spectrometry within Drug Discovery
Mattias Rohman and Jonathan Wingfield

4. Structure-Based Virtual Screeningof Commercially Available Compound Libraries
Dmitri Kireev

5. AlphaScreen-Based Assays:Ultra-High Throughput Screening for Small Molecule Inhibitors of ChallengingEnzymes and Protein-Protein Interactions
Adam Yasgar, Ajit Jadhav, AntonSimeonov, and Nathan P. Coussens

6. Instrument Quality Control
Chatura Jayakody and Emily A.Hull-Ryde

7. Application of FluorescencePolarization in HTS Assays<Xinyi Huang and Ann Aulabaugh

8. Time Resolved Fluorescence Assays
Chen-Ting Ma and Eduard A. Sergienko

9. Protein Kinase SelectivityProfiling Using Microfluid Mobility Shift Assays
Peter Drueckes

10. Screening for Inhibitors ofKinase Autophosphorylation
Bianca Heedmann and Martin Klumpp

11. A Fluorescence-Based HighThroughput Screening Assay to Identify Growth Inhibitors of the PathogenicFungus Aspergillus fumigatus
Thomas M. Smith, Daryl L. Richie,Jianshi Tao

12. Mycobacterium tuberculosisHigh Throughput Screening
E. Lucile White, Nichole A. Tower,and Lynn Rasmussen

13. Identification ofState-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR BasedAssay
Alberto di Silvio

14. A Luciferase Reporter GeneSystem for High Throughput Screening of γ-Globin GeneActivators
Wensheng Xie, Robert Silvers, MichaelOuellette, Zining Wu, Quinn Lu, Hu Li, Kathleen Gallagher, Kathy Johnson, andMonica Montoute

15. A High Throughput Flow CytometryAssay for Identification of Inhibitors of 3’, 5’-Cyclic Adenosine MonophosphateEfflux
Dominique Perez, Peter C. Simons,Yelena Smagley, Larry A. Sklar, and Alexandre Chigaev

16. High Throughput Cell ToxicityAssays
David Murray, Lisa McWilliams, andMark Wigglesworth

17. BRETn: NanoLuc-BasedBioluminescence Resonance Energy Transfer Platform to Monitor Protein-ProteinInteractions in Live Cells
Xiu-Lei Mo and Haian Fu

18. Application of Imaging-BasedAssays in Microplate Formats for High Content Screening
Adam I. Fogel, Scott E. Martin, andSamuel A. Hasson


Textul de pe ultima copertă

This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening,  high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.

Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts