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Heterologous Expression of Membrane Proteins: Methods and Protocols: Methods in Molecular Biology, cartea 601

Editat de Isabelle Mus-Veteau
en Limba Engleză Hardback – 18 dec 2009
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.
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Specificații

ISBN-13: 9781607613435
ISBN-10: 1607613433
Pagini: 276
Ilustrații: XI, 272 p.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.68 kg
Ediția:2010
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Descriere

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.

Cuprins

Heterologous Expression of Membrane Proteins: Methods and ProtocolsIsabelle Mus-Veteau, Editor Table of ContentsPrefaceContributors1. Heterologous expression of membrane proteins for structural analysis Isabelle Mus-Veteau2. Production of membrane proteins in E. coli and L. lactisBert Poolman and Eric R. Geertsma3. Mammalian membrane receptors expression as inclusion bodies in E. coliBernard Mouillac and Jean-Louis Banères 4. Expression of membrane proteins at the E. coli membrane for structural studiesManuela Zoonens and Bruno Miroux5. Membrane protein expression in Lactococcus lactisAnnie Frelet-Barrand, Sylvain Boutigny, Edmund R. S. Kunji and Norbert Rolland6. Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiaeOlivier Joubert, Rony Nehme, Michel Bidet and Isabelle Mus-Veteau7. Mammalian membrane proteins expression in baculovirus infected insect cellsCéline Trometer and Pierre Falson8. Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G Protein-Coupled Receptor as a modelKarl Brillet, Carlos A. Pereira, and Renaud Wagner9. Membrane protein expression in the eyes of transgenic flies.Valérie Panneels and Irmgard Sinning10. Expression of Mammalian Membrane Proteins in Mammalian Cells using Semliki Forest Virus VectorsKenneth Lundstrom11. Membrane Protein Expression in Cell-Free SystemsBirgit Schneider, Friederike Junge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dötsch, Frank Bernhard12. Practical considerations of membrane proteininstability for purification and crystallisation Christopher G. Tate13. Membrane protein solubilizationKatia Duquesne and James N. Sturgis 14. Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitroCécile Breyton, Bernard Pucci and Jean-Luc Popot15. Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPaseDelphine Cardi, Cédric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc le Maire and Christine Jaxel

Recenzii

From the reviews:
“Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. … Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer … . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research.” (Omer Iqbal, Doody’s Review Service, July, 2010)

Textul de pe ultima copertă

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. 

Caracteristici

Provides descriptions of existing heterologous expression systems for membrane proteins and instructions for manipulating these systems
Contains methods for the purification of membrane proteins
Includes information on classical detergents and alternatives for studying membrane proteins in solution
Offers methods to study and increase membrane proteins stability
Includes supplementary material: sn.pub/extras