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Fluorescence Spectroscopy and Microscopy: Methods and Protocols: Methods in Molecular Biology, cartea 1076

Editat de Yves Engelborghs, Antonie J.W.G. Visser
en Limba Engleză Hardback – 10 oct 2013
Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
 
Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
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Specificații

ISBN-13: 9781627036481
ISBN-10: 1627036482
Pagini: 840
Ilustrații: XXI, 816 p. 244 illus., 171 illus. in color.
Dimensiuni: 178 x 254 x 50 mm
Greutate: 1.68 kg
Ediția:2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Part I: Steady-State Fluorescence Spectroscopy (SSFS)
 
1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
            Adam Matthew Gilmore
 
2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
            Nicholas G. James and David M. Jameson
 
3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation
            Ivo H.M. van Stokkum and Sergey P. Laptenok
 
4. High-Pressure Fluorescence Applications
            Mariano Dellarole and Catherine A. Royer
 
Part II: Time-Resolved Fluorescence Spectroscopy (TRFS)
 
5. Frequency Domain Fluorometry: Theory and Application
            Carissa M. Vetromile and David M. Jameson
 
6. Polar Plot Representation of Time-Resolved Fluorescence
            John Paul Eichorts, Kai wen Teng, and Robert M. Clegg
 
7. Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics
            Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, and Elisha Haas
 
8. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
            Patrik R. Callis and Jose R. Tusell
 
9. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins
            Dmitri Toptygin
 
10. Global Analysis of Time-Resolved Fluorescence Data
            Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, and Vladimir V. Apanasovich
 
11. Nanometrology
            David J.S. Birch and Philip Yip
 
12. Upconversion Spectrophotofluorometry
            Arianna Biesso, Jianhua Xu, and Jay R. Knutson
 
13. Subpicosecond Kerr-Gate Spectrofluorometry
            Sergey P. Laptenok, Patrick Nürnberger, Andras Lukacs, and Marten H. Vos
 
14. Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay
            Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, and Fumio Tanaka
 
Part III: Fluorescent Probe Development (FPD)
 
15. Biosynthetic Incorporation of Trp Analogs in Proteins
            Jaap Broos
 
16. Optimization of Fluorescent Proteins
            Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, and Theodorus W.J. Gadella, Jr.
 
17. Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family
            Zeinab Darwich, Andrey S. Klymchenko, and Yves Mély
 
Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP)
 
18. Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope
            Ranhua Xiong, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt, and Kevin Braeckmans
 
Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim)
 
19. A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging
            Clemens F. Kaminski, Eric. J. Rees, and Gabriele S. Kaminski Schierle
 
Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM)
 
20. Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors
            Roland Hartig, Yury Prokazov, Evgeny Turbin, and Werner Zuschratter
 
21. Global Analysis of FRET- FLIM Data in Live Plant Cells
            Sergey P. Laptenok, Joris J. Snellenburg, Christoph A. Bücherl, Kai R. Konrad, and Jan Willem Borst
 
22. Time-Resolved Fluorescence Anisotropy Imaging
            Klaus Suhling, James Levitt, and Pei-Hua Chung
 
23. Multi-Modal Fluorescence Imaging Spectroscopy
            Martijn H.W. Stopel, Christian Blum, and Vinod Subramaniam
 
Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS)
 
24. Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells
            Thomas Weidemann
 
25. Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells
            Xiaoxiao Ma, Yong Hwee Foo, and Thorsten Wohland
 
26. Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS)
            Ana M. Melo, Manuel Prieto, and Ana Coutinho
 
27. PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching
            Markus Sauer and Hannes Neuweiler
 
28. Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes
            Tomáš Steinberger, Radek Macháň, and Martin Hof
 
29. Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane
            Paul Müller, Petra Schwille, and Thomas Weidemann
 
30. Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS
            Jelle Hendrix and Don C. Lamb
 
31. Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy
            Sergi Padilla-Parra, Nicolas Audugé, Maïté Coppey-Moisan, and Marc Tramier
 
32. Brightness Experiments
            Patrick J. Macdonald, Jolene Johnson, Yan Chen, and Joachim D. Mueller
 
33. Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy
            Victor V. Skakun, Anatoli V. Digris, and Vladimir V. Apanasovich
 
34. Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy
            Igor P. Shingaryov, Victor V. Skakun, and Vladimir V. Apanasovich
 
Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS)
 
35. Single-Molecule Fluorescence of Nucleic Acids
            Kaley McCluskey, Euan Shaw, Daniel A. Lafontaine, and J. Carlos Penedo
 
36. Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light
            Susana Rocha, Herlinde De Keersmaecker, Hiroshi Uji-i, Johan Hofkens, and Hideaki Mizuno

Recenzii

From the reviews:
“The book is illustrated with high-quality figures in both color and black and white. … This book offers techniques from internationally recognized experts. Both novice and established scientists will find it extremely useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2014)

Textul de pe ultima copertă

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
 
Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Caracteristici

Explores the methods and applications in the expanding fluorescence field
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts