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Clostridium difficile: Methods and Protocols: Methods in Molecular Biology, cartea 646

Editat de Peter Mullany, Adam P. Roberts
en Limba Engleză Hardback – 8 iun 2010
Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.
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Specificații

ISBN-13: 9781603273640
ISBN-10: 1603273646
Pagini: 241
Ilustrații: X, 241 p.
Dimensiuni: 178 x 254 x 16 mm
Greutate: 0.65 kg
Ediția:2010
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Descriere

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.

Cuprins

Part I: Introduction to C. difficile and the Disease It Causes1. Clostridium difficile: No Longer an Enigmatic Pathogen?Adam P. Roberts and Peter Mullany2. Clostridium difficile and the Disease It CausesTorbjörn NorénPart II: Isolation and Culture Techniques3. Clostridium difficile Isolation and Culture TechniquesMike WrenPart III: Methods for Typing and Epidemiological Studies4. Molecular Typing Methods for C. difficile: Pulsed-Field Gel Electrophoresis and PCR RibotypingSandra Janezic and Maja Rupnik5. Clostridium difficile ToxinotypingMaja Rupnik6. Multilocus Sequence Typing for Clostridium difficileLudovic Lemée and Jean-Louis PonsPart IV: Biochemistry of the Organism7. Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin GenesAna Antunes and Bruno Dupuy8. Dissecting the Cell SurfaceRobert Fagan and Neil Fairweather9. Human Intestinal Epithelial Response(s) to Clostridium difficileNazila V. Jafari, Elaine Allan, and Mona Bajaj-ElliottPart V: Genomics10. Comparative Genome Analysis of Clostridium difficile Using DNA MicroarraysRichard Stabler, Lisa Dawson, and Brendan WrenPart VI: Development of Systems for Genetic Analysis of the Organism11. ClosTron-Targeted MutagenesisJohn T. Heap, Stephen T. Cartman, Sarah A. Kuehne, Clare Cooksley, and Nigel P. Minton12. Methods for Gene Cloning and Targeted MutagenesisGlen P. Carter, Dena Lyras, Rachael Poon, Pauline M. Howarth, and Julian I. Rood13. Transposon Mutagenesis in C. difficileHaitham A. Hussain, Adam P. Roberts, Rachael Whalan, and Peter MullanyPart VII: Animal Models ofDisease14. Refinement of the Hamster Model of Clostridium difficile DiseaseGillian Douce and David Gouding15. Methods for Working with the Mouse ModelAnne Collignon

Recenzii

From the reviews:
“This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea after prolonged antibiotic treatment. … This book would be useful to scientists working with bacteria such as C. difficile that cause toxin-mediated diseases in the colon of their host. … This is a technical book that provides essential methods and techniques to study devastating diseases due to Clostridium difficile.” (Rebecca T. Horvat, Doody’s Review Service, October, 2010)

Textul de pe ultima copertă

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.

Caracteristici

Serves as a comprehensive guide to most of the current techniques for manipulating and analyzing C. difficile
Written by scientists who have pioneered modern C. difficile research
Brings together in one place chapters from clinicians, clinical laboratory scientist, and basic scientists to provide a comprehensive understanding of the practical state of the art in C. difficile research
Includes supplementary material: sn.pub/extras