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Capillary Electrophoresis of Biomolecules: Methods and Protocols: Methods in Molecular Biology, cartea 984

Editat de Nicola Volpi, Francesca Maccari
en Limba Engleză Hardback – 6 feb 2013
Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.
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Specificații

ISBN-13: 9781627032957
ISBN-10: 1627032959
Pagini: 372
Ilustrații: XIV, 358 p.
Dimensiuni: 178 x 254 x 26 mm
Greutate: 0.91 kg
Ediția:2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

1. Recent Advances in Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery
Chenchen Wang, Xueping Fang, and Cheng S. Lee
2. Capillary Electrophoresis-mass Spectrometry of Carbohydrates
Joseph Zaia
3. Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis
M.C. Breadmore
4. Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis
Mitsuru Abo, Li-ping He, Kae Sato and Akira Okubo
5. Determination of Mono-Di-and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contractless Conductivity Detection
Claudimir Lucio do Lago, Thiago Nogueira, Lucas Blanes, and Renata Mayumi Saito
6. Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis
Toshiaki HATTORI, Nobuhiro ANRAKU and Ryo KATO
7. Capillary Electrophoresis for the Analysis of Glycosaminoglycan-derived Disaccharides
Yuqing Chang, Bo Yang, Amanda Weyers, Robert J. Linhardt
 8. High Throughput Capillary Electrophoresis Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes
Mehdi Moini
9. Separation of Amino Acids by Capillary Electrophoresis with Light-emitting Diode-induced Fluorescence in the Presence of Electroosmotic Flow
Ming-Mu Hsieh, Po-Ling Chang
 10. Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-amplified Sample Injection Capillary Electrophoresis UV Detection
Angelo Zinellu, Salvatore Sotgia, Luca Deiana,  Ciriaco Carru
11. Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies
Carolina Simo, Alejandro Cifuentes, Václav Kašička
 12. High Resolution Proteome/peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS
Amaya Albalat, Vasiliki Bitsika, Petra Zurbig, Justyna Siwy, and William Mullen
13. Contribution of CE to the Analysis of Protein or Peptide Biomarkers
Kiarach Mesbah, Romain Verpillot, François de l’Escaille, Jean Bernard Falmagne, Myriam Taverna
 14. Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis
R. Nehmé and C. Perrin
15. Capillary Electrophoresis with Laser Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids
Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, Andras Guttman, Jose Carlos Diez-Masa, Mercedes de Frutos
 16. Automated Capillary Electrophoresis in the Screening
for Haemoglobinopathies

Frédéric Cotton, Fleur Wolff, Béatrice Gulbis
17. Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-capillary Derivatization and Laser-induced Fluorescence Detection
Cristina Pelaez-Lorenzo, Maria Teresa Veledo, Ramon Gonzalez, Mercedes de Frutos, Jose Carlos Diez-Masa
 18. Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits
Tomoyuki Katsube-Tanaka
19. Evaluating Amyloid Beta (Ab) 1-40 Degradation by Capillary Electrophoresis
Benjamin J. Alper and Walter K. Schmidt
 20. Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei
Julie M. Chandler, Heather R. Trenary, Gary R. Walker, and, Jr.
 21. Capillary Electrophoretic Enzyme Assays
Gerhard K. E. Scriba, Hans Abromeit, Martina Hense and Yi Fan
22. A Methodology for Detection and Quantification of Esterase Activity
Ana L. Simplício, Ana S. Coroadinha, John F. Gilmer and Joana Lamego
 23. Enzyme Inhibitor Screening by CE with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique
Chao Liu, Qianqian Zhang, Jingwu Kang
 24.  Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis
Philip M. Yangyuoru, Latanya Hammonds-Odie, Simon M. Mwongela
25. Assessment of DNA damage by Micellar Electrokinetic Chromathography
Paolo Fattorini, Giorgio Marrubini, Pierangela Grignani, Solange Sorçaburu-Cigliero, Carlo Previdere`

Textul de pe ultima copertă

Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.

Caracteristici

Focuses on experimental methods for the characterization of carbohydrates, mono-, oligo-, and polysaccharides
Presents current, up-to-date methodologies
Features tips on troubleshooting and avoiding known pitfalls
Includes supplementary material: sn.pub/extras