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Calcium Signaling Protocols: Methods in Molecular Biology, cartea 937

Editat de David G. Lambert, Richard D. Rainbow
en Limba Engleză Paperback – 23 aug 2016
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.
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Specificații

ISBN-13: 9781493959280
ISBN-10: 149395928X
Pagini: 374
Ilustrații: XI, 360 p.
Dimensiuni: 178 x 254 x 20 mm
Greutate: 6.98 kg
Ediția:Softcover reprint of the original 3rd ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Part I: General
1. Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations
            Alec W.M. Simpson
2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
            Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert
Part II: Specialist Measurement Systems
3. Confocal Microscopy: Theory and Applications for Cellular Signaling
            Stephen C. Tovey, Paul J. Brighton, Edward T.W. Bampton, Yan Huang, and Gary B. Willars
4. Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer
            Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty
5. Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®)
            Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty
6. Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader
            Benjamin D. Hunt and David G. Lambert
Part III: Measurement of Ca2+ Channel Activity
7. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones
            Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, and Andrew D. Randall
8. Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells
            Richard D. Rainbow
Part IV: Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores
9. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells
            Alison Skippen, Philip Swigart, and Shamshad Cockcroft
 
10. Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells
            Carl P. Nelson
11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
            Darren Smart
Part V: Specialist Measurement Techniques
12. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
            Hideo Kanaide and Katsuya Hirano
13. Calcium Measurements from Whole Heart Using Rhod-2
            Bum-Rak Choi
14. Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries
            Kim A. Dora and Michael A. Hill
15. Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration
            John G. McCarron, Marnie L. Olson, Susan Chalmers, and John M. Girkin
16. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
            Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch
17. Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes
            Denis Ottolini,Tito Calì, and Marisa Brini
18. Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors
            Valeria Camarda and Girolamo Calo
19. Compartmentalizing Genetically-Encoded Calcium Sensors
            David A. Williams, Mastura Monif, and Kate L. Richardson
20. Measurement of Cytosolic-Free Ca2+ in Plant Tissue
            Martin R. McAinsh and Carl K.-Y. Ng
21. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)            Danuta Kosk-Kosicka

Textul de pe ultima copertă

The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.

Caracteristici

Combines brand new protocols with heavily revised and improved methodologies
Features lab-ready procedures with step-by-step instruction
Includes expert tips and key implementation advice to ensure successful results
Includes supplementary material: sn.pub/extras