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Biological Aging: Methods and Protocols: Methods in Molecular Biology, cartea 1048

Editat de Trygve O. Tollefsbol
en Limba Engleză Paperback – 30 apr 2017
The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.
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Specificații

ISBN-13: 9781493963133
ISBN-10: 1493963139
Pagini: 354
Ilustrații: XI, 354 p. 54 illus., 35 illus. in color.
Dimensiuni: 178 x 254 x 19 mm
Greutate: 0.64 kg
Ediția:Softcover reprint of the original 2nd ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

1. Cell Senescence Culturing Methods
Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol
 
2. Digital Image Analysis of Cells Stained with the Senescence-Associated Beta-Galactosidase Assay
Liran I. Shlush and Sara Selig
 
3. Analysis of Biomarkers of Caloric Restriction in Aging Cells
Yuanyuan Li and Trygve O. Tollefsbol
 
4. Cell Sorting of Young and Senescent Cells
Graeme Hewitt, Thomas von Zglinicki, and João F. Passos
 
5. Studying the Replicative Life Span of Yeast Cells
David A. Sinclair
 
6. Methods for Creating Mutations in C. elegans that Extend Lifespan
Dayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong
 
7. Aging Studies in Drosophila Melanogaster
Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou
 
8. The Use of Calorie Restriction Mimetics to Study Aging
Munehiro Kitada and Daisuke Koya
 
9. Using Somatic-Cell Nuclear Transfer to Study Aging
Satoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama
 
10. Induction of Cellular Senescence by Oncogenic RAS
Romi Gupta and Narendra Wajapeyee
 
11. Methods of Cellular Senescence Induction Using Oxidative Stress
Zhe Wang, Dandan Wie, and Hengyi Xiao
 
12. Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice
 Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski
13. Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors
Dejia Li and Dongsheng Duan
 
14. Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration
Nadav Kimelman Bleich, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli , Zulma Gazit , Gadi Pelled, and Dan Gazit
15. Exometabolomic Mapping of Caenorhabditis elegans – A Tool to Non-Invasively Investigate Aging
Robert J. Mishur, Jeffrey A. Butler, and Shane L. Rea
 
16. Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing
Gilberto Vargas-Alarcón and Carmina Flores-Domínguez
 
17. Gel Electrophoresis-based Proteomics of Senescent Tissues
Steven Carberry and Kay Ohlendieck
 
18.  Single-Cell Semiconductor Sequencing
Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz
 
19. Application of DNA Microarray Technology to Gerontological Studies
Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan
 20. Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells Carmen M. Koch and Wolfgang Wagner
 
21. Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging
Leonid L. Moroz  and Andrea B. Kohn
 

Textul de pe ultima copertă

The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.

Caracteristici

Focuses on contemporary approaches and established protocols to study aging cell culture
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras

Descriere

Descriere de la o altă ediție sau format:
Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.

Recenzii

From the reviews:
"For research gerontologists, this book is a boon." -Doody's Book Review, Weighted Numerical Score:90 - 4 Stars
"This can be a useful book for biogerontologists. … Comprised of 25 generally well-written articles … this book covers various methods and protocols for studying cellular aging of human cells, yeast cells, and mice. … a welcome source of useful information for researchers intending to find their way into descriptive, analytical and interventive research in biogerontology." (Suresh Rattan, Biogerontology, Issue 9, 2008)